Question

关于基因敲除现在要做酵母的URE2基因敲除 目的基因为一抗性基因1、酵母的URE2基因是什么?2、PCR出来的目的基因如

Answer 1

1. Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion

http://www.***.org/cgi-bin/locus.fpl?locus=ure2

2. http://home.cc.umanitoba.ca/~gietz/method.html

3. i suppose so．．