In the research of molecular biology, The sequence analysis of DNA is On the basis of
further researching and reformming of destination gene sequencing , even Test sequence is the essential link of equence analysis . The most widely used sequencing is dideoxy chain termination method. This study relates to a new DNA sequencing technology called" alpha-thiosequencing" , the technology is mainly used to remove the exonuclease extending fluorescence signal and the fact that alpha nucleotides extends the thiosulfate. The amplification product is fixed on the solid substrate, removing the complementary strand degeneration after hybridization, the phosphorothioate modified sequencing primers, containing single known nucleotide monomer mixture ( fluorescently labeled with normal nucleotide monomers ) extension reaction solution is formed extending reaction nucleosides keys via exonuclease III, the3 ' - > 5' exonuclease removal of the fluorescent activity extends base; extension has the same type of thiosulfate nucleotide monomers, so that the formation of phosphorothioate nucleosides keys, antagonizes the next enzyme reaction, to ensure that the primer extends forward, with second known nucleotide monomer extension reaction solution extension reaction. According to the principle of complementary base pairing, only joining the nucleotide monomers and then extending the base template matching, extending the reaction can be carried out, therefore, the four base pairs ( A, T, C, G ) in turn, can eventually template sequence base information. The method will provide people the life course research, molecular mechanisms of genomic DNA sequence analysis, individualized medicine, disease prediction and prevention, drug development and individualized study has greater application value.