硼酸-硼砂缓冲液(0.2M硼酸根)
pH 0.05M硼砂/ml 0.2M硼酸/ml pH 0.05M硼砂/ml 0.2M硼酸/ml
7.4 1.0 9.0 8.2 3.5 6.5
7.6 1.5 8.5 8.4 4.5 5.5
7.8 2.0 8.0 8.7 6.0 4.0
8.0 3.0 7.0 9.0 8.0 2.0
硼砂Na2B4O7•10H2O分子量=381.43 0.05M(=0.2M硼酸根)溶液为19.07g/L
硼酸H3BO3分子量=61.84 0.2M溶液为12.37g/L
硼砂易失去结晶水,必须在带塞的瓶中保存
硼酸-氯化钾-氢氧化钠缓冲液
PH8.0
50ml0.1mol/L氯化钾-硼酸缓冲液,加入3.9ml0.1mol/L氢氧化钠和46.1ml水终体积100ml