ph=6.3和ph=6.5的磷酸盐缓冲液怎样配置?

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  • 磷酸盐缓冲液

    磷酸氢二钠-磷酸二氢钠缓冲液(0.2M)

    pH 0.2M Na2HPO4/ml 0.2M NaH2PO4/ml

    5.8 8.0 92.0

    5.9 10.0 90.0

    6.0 12.3 87.7

    6.1 15.0 85.0

    6.2 18.5 81.5

    6.3 22.5 77.5

    6.4 26.5 73.5

    6.5 31.5 68.5

    6.6 37.5 62.5

    6.7 43.5 56.5

    6.8 49.5 50.5

    6.9 55.0 45.0

    7.0 61.0 39.0

    7.1 67.0 33.0

    7.2 72.0 28.0

    7.3 77.0 23.0

    7.4 81.0 19.0

    7.5 84.0 16.0

    7.6 87.0 13.0

    7.7 89.5 10.5

    7.8 91.5 8.5

    7.9 93.0 7.0

    8.0 94.7 5.3

    Na2HPO4•2H2O分子量=178.05 0.2M溶液含35.61g/L

    Na2HPO4•12 H2O分子量=358.22 0.2M溶液含71.64g/L

    NaH2PO4•H2O分子量=138.01 0.2M溶液含27.6g/L

    NaH2PO4•2H2O分子量=156.03 0.2M溶液含31.21g/L赞同3| 评论(2)