D 此题考查固定搭配和定语从句.
which作为关系代词,等同于先行词the microscope,放入定语从句中为：
with which we had so much trouble
= we had so much trouble with which
= we had so much trouble with the microscope
此句含有固定搭配：have trouble/difficulty with sth,意思是“在某事上有麻烦/困难”.
已经讲得很清楚啦
英语语法This is the microscope ______ which we have had so much
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