我不擅长改错,我替你重新整理来一次不知道是否合用：
i am sorry for my misbehaviors of these days.recently i felt so depressed due to breakup with my boyfriend who is an american and the relationship had lasted about 2 years.I was so
sad for those moments.Now I came to myself from this terrible strike.Don't worry about me .Friends of mine conforted and inspired me through all these horrible times.Now I stood up on own feet facing future optimistically
（I am sorry for my previous behaivor.i depressed resently,Never thought the boyfriend of 2 years who is in American suddenly broke up with me,so I am sorry,but don't worry,After friend's encouraging and enlightenning me,now I am ok and I pluck up the courage to face it now.optimistically ）
i am writing this log to record down what and how i had done in the past 3 months.I cultured HCT 116 cells and counted them with the help of CASY.Then I helped Sarah with her other cell culturing and made some sections in other labs.In the second month,I practiced Western blot on PGK1 and Beta-actin.For the first time the line 3 had no trace of PGK1,
contradictory to my former results.So I figured that the concentration of proteins might be too low.In the experiment I used just 100ug（浓度,还是净含量）.I was not sure whether i had made a mistake by detecting the content of protein or else.Maybe I ought to titrate PGK1 twice and add more protein for next action..It was not very good for Beta-actin,I couldn't find why.Sarah said this might due to less protein ,But I tested it with the R-T PCR and the result was ok.
I am writing to explain what I have done in the last 3 months.In the first month,I cultured HCT 116 cells and counted them with the help of CASY.Then I helped Sarah culture other cells and made sections in the other lab.In the second month,I practiced Western blot on PGK1 and Beta-actin.At the first time the line 3 had no trace for PGK1,I did it in the same way ,so i thought proteins might be not enough,i just used 100ug.I am not sure whether i have made a mistake detecting the content of protein.Maybe I need to measure protein twice and add more protein.It was not every good for Beta-actin,I did not know why,Sarah said it maybe owe to less protein,I did the R-T PCR as well and the result was ok.
during the last month,I still carried on the cell cultivation ,western blot and R-T PCR.We did western blot ,me on the beta-actin meanwhile my partner on PGK1,we used the same quantity of proteins which are different and from differing cell lines.The result on Beta-actin was unsatisfactory.By analysis I concluded we might need more protein to detect Beta-actin in western blot .And also I sampled a lot of sections of tumors as well as nomal tissues.I repeated R-T PCR ,although the last sample was not very satisfactory as a result of not having enough solutions supplies.
In the last month,I continued to culture cells and do western blot and R-T PCR.I did western blot on beta-actin and another person did it on PGK1,we used the same quality of protein but different protein of cells,however the result on Beta-actin was bad.By which I think we need more protein to do western blot on Beta-actin.I made a lot of sections of tumors or nomal tissues.I did R-T PCR again although the last sample is not very good for we didn't have enough solutions as we needed in our lab.
Whatelse,I have been continuing my english studies in my leisure time.I planned to take
immnohistochemical experiments after this vacation.
Here I would like to express my aboudance of thanks for you and your helps..For everything you have done to me is highly appreciated.Wishing you all the best in the coming year and the ever .
In addition,I am continuing study english in my spare time and planning to do immnohistochemical experiments after the vacation.I would very much like to thank you for your help and everything you have done for me is greatly appreciated.Wishing you all the best in the coming new year and as always.（最后这段分成2部分表达较好）