正好看到,匆匆翻译了一下.要是有疏漏,提醒一下,呵呵.
Gel electrophoresis is a popular method to analyze protein mixture, measure the relative amount of protein or peptide species. It is also a powerful biochemical analysis tool to detect protein purity and its molecular weight. PAGE gel staining after electrophoresis is a critical step. Coomassie stain is one of the most popular methods. It has the following advantages: First of all, low cost for the reagents and also does not require expensive scanning equipment. Secondly, many protocols can be chosen from to fit the experimental requirement to balance between simplicity and sensitivity. Last but not the least, it is also well compatible with the mass spectrometry. The current experiment compared several Coomassie stain protocols that used in DNA binding protein electrophoresis assay.