In recent years, oil pollution is serious, how to fix the contaminated soil and the study of the method of increasingly become everyone's attention, and high efficiency degradation bacterium hydrocarbon screening is one of the big popular one. This article through to the laboratory of 3 kinds of petroleum hydrocarbon preservation degradation bacterium (Z1, Z41, Z49) preliminary determination, to select the degradation effect is good oil strains, and then in order to further improve the efficiency of the fungus oil degradation, and carry on the uv mutation breeding, induced time is divided into 5 s, 15 s, 30 s, 40 s, 60 s, again through the blood flat screen and wave at the beginning of the bed after screen, selected high efficiency degradation of the strain, the strain of eventually get V23 degradation rate was 42.07%, up 20.07% before and contrast of the several candidates in activity can watch strains of evaluation, found that the strains of surface active agent production was greatly enhanced, and one of the 2 μ L V23 diluted 10 times the fermented liquid so clear 37.14 mm circle, rat lee sugar fat 19.6 g/L, a strain on up by more than 31%.
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