Overall,these analysis based 3D graphic surfaces were accorded with results of single factor experiments showed Figs.1–4.Then the prediction was obtained by DPS.Although the prediction showed that the more immersing time and the lower solid:liquid ratio were,the higher yield of polysaccharides,a low solid:liquid ratio 1:67 and a less immersing time 10 min were selected in this study.It is considered the effect for yield of polysaccharides in independent variable of immersing time was trivial,and longer immersing time will lead lower efficiency.
整体而言,这些已经过分析的三维图形表面与.图示1–4所显示的单因子实验结果是一致的; 然后通过DPS数据处理系统获得预测.该预测显示,随着浸泡时间的增长和固液比率的降低,多糖的收率就越高.本研究所选择的是较低的固液比率,只有1:67,以及较短的浸泡时间,10分钟；因为浸泡时间的自变量对于多糖收率的作用被认为是微不足道的,而更长的浸泡时间反而会导致效率下降.
So,based on the results in Fig.5–7,a optimum condition was found as follow:solid:liquid ratio of 1:67,immersing time of 10 min,water extraction temperature of 95 _C,and water extraction time of 15 min,then the highest polysaccharides yield of 11.36% predicted by the contour plots could be obtained.Maybe,a more liquid volume can rapidly dissolve more polysaccharides from sample cells in the same high temperature than little volume,the dissolved polysaccharides reached to be saturated in a definite extraction time,and then the polysaccharide was obtained the highest yield.The calculated yield was in agreement with the four experimental mean values of 11.29%.
因此,根据图示5–7所显示的结果,最佳的工艺条件如下：固液比率 1:67、浸泡时间10分钟、水提温度95°C、水提时间15分钟；按此条件,即可获得等高图所预测的11.36% 最高多糖收率.也许,以同样的温度增加水量可以很快地从细胞样本溶解出更多的多糖,经过确切的水提时间的浸泡,可以获得更高的多糖收率.计算出的收率与四个实验的平均值11.29% 是一致的.
In this study,application of Uniform Design in the extraction from Gynostemma pentaphyllum Makino improved yields of polysaccharides and was less extraction time than conventional heating.
本研究通过使用均匀设计法从绞股蓝提取多糖可获得更高的收率,而且水提时间比常规加热方式所需的时间更短.
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