通常大赛璐手性商品柱的流动相建议使用流速0.5mL/min,但有的时候根据需要也可以调整至1.0mL/min.置于你这个应该是0.5mL/min.
紫外检测器波长254nm.
洗脱时间分别是7.280min和8.532min.
t0是需要检测人员给的,然后计算分离因子α.
如果是寄出去的样品在外边测的,最好问问人家具体的检测条件,这些应该是给出来的.
求帮忙读一下这个色谱图The ee of product was de
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