Question

求SDS-PAGE 配方 分离胶 7.5% 浓缩胶 4%

Answer 1

5ml 7.5%分离胶：

H2O 2.35ml

30%丙烯酰胺溶液 1.25ml

1.5M Tris(pH8.8) 1.3ml

10%SDS 50ul

10%APS 50ul

TEMED 4ul

5ml 4%浓缩胶：

H2O 3ml

30%丙烯酰胺溶液 670ul

0.5M Tris(pH6.8) 1.25ml

10%SDS 50ul

10%APS 50ul

TEMED 4ul